Experimental and Data Analysis Workflow for Soft Matter Nanoindentation
نویسندگان
چکیده
Nanoindentation refers to a class of experimental techniques where micrometric force probe is used quantify the local mechanical properties soft biomaterials and cells. This approach has gained central role in fields mechanobiology, design tissue engineering, obtain proper characterization materials with resolution comparable size single cells (μm). The most popular strategy acquire such data employ an atomic microscope (AFM); while this instrument offers unprecedented (down pN) space (sub-nm), its usability often limited by complexity that prevents routine measurements integral indicators properties, as Young's Modulus (E). A new generation nanoindenters, those based on optical fiber sensing technology, recently popularity for ease integration allowing apply sub-nN forces µm spatial resolution, therefore being suitable hydrogels In protocol, step-by-step guide detailing procedure nanoindentation using commercially available ferrule-top nanoindenter presented. Whereas some steps are specific herein, proposed protocol can be taken other devices, granted adapted according manufacturer's guidelines. Further, open-source Python software equipped user-friendly graphical user interface analysis presented, which allows screening incorrectly acquired curves, filtering, computation contact point through different numerical procedures, conventional E, well more advanced particularly suited single-cell data.
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متن کاملProvenance and data differencing for workflow reproducibility analysis
One of the foundations of science is that researchers must publish the methodology used to achieve their results so that others can attempt to reproduce them. This has the added benefit of allowing methods to be adopted and adapted for other purposes. In the field of e-Science, services – often choreographed through workflow, process data to generate results. The reproduction of results is ofte...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2022
ISSN: ['1940-087X']
DOI: https://doi.org/10.3791/63401-v